Indlela neutralization, eli yendlela isicelo esisebenzayo

igama elithi "neutralization reaction" ezikhoyo chemistry ezingaphiliyo luthetha inkqubo imichiza apho izinto basabela ngempahla emuncu kunye ezingundoqo, oko kwaba neziphumo zokulahlekekelwa-nxaxheba bayavuma kunye nabo iikhemikhali kunye nezinye iipropati isici. Neutralization kwi eziphilayo unalo intsingiselo efanayo kwihlabathi iimveliso zayo balahlekelwe iipropati zabo omzalayo. Kodwa, Kakade, yinkqubo eyahlukileyo ngokupheleleyo kunye nabanye abathathi-nxaxheba kunye nesiphumo. Kwaye ngokobugcisa, ngawo kwaye ikakhulu amagqirha abanomdla kunye izazinzulu - na ukukwazi a silwanyana okubangela izifo okanye ukufa isilwanyana sifo.

Ngoko ke, yintoni na? Neutralization reaction - isetyenziswa elebhu kwesifo test serological, apho izilwa-sera omzimba anqanda umsebenzi ngeentsholongwane, kwakunye ukukhutshwa eziyityhefu kunye nezinto eziphilayo esebenzayo (enzymes).

izicelo

Amaninzi, le ndlela yophando esetyenziswayo ukwazisa intsholongwane, oko kukuthi, izifo egazini ezosulelayo. Ngaphezu koko, uvavanyo ingabhekiswa kwi kokubini ukuphawulwa pathogen, kunye antibodies noko.

In ngeebhakther le ndlela isetyenziswa ngokuqhelekileyo xa efumanise omzimba ukuba enzyme iintsholongwane ezifana antistreptolysin, antistafiloliziny, antistreptokinazy.

kwenziwa njani uvavanyo

indlela neutralization isekelwe ekukwazini lomzimba - iiproteni igazi athile omzimba - lwaze ezisegazini - arhente zasemzini ufake umzimba. Ukuba Ubhaqo pathogen kuyimfuneko kunye nokuchonga, ngoko ugalelwe serum eqhelekileyo omzimba omzimba kunye izinto eziphilayo. Lo mxube yayigcinwe ngesifukamisi ngexesha elifanelekileyo kwaye liqalise zibe zezinto eziphilayo sifo. Ezi zizilwanyana laboratory (iimpuku, iimpuku), iseli umbungu inkcubeko phuphu. Xa engekho eziphilayo isiphumo (ukugula okanye ukufa wesilwanyana), siyakwazi ukubona ukuba lo intsholongwane, a serum standard apho kusetyenziswa khona. Ekubeni, njengokuba kuthiwe, ibonisa ukuba indlela lwenzekile, kukuphelelwa bale ntsholongwane biosvoystv (ukukwazi ukubangela nokufa kwesilwanyana) ngenxa impefumlelwano izilwa-serum kunye awakwazi. Xa kumiselwa izinto eziyityhefu algorithm yezenzo iyafana, kodwa zikhona ezinye iindlela. Xa uhlola nayiphi zeendawana equlathe yoluvo, ngoko ke ixutywe kunye serum eqhelekileyo. Kwimeko isifundo yokugqibela wasebenzisa ulawulo kweziyobisi ityhefu. Waya indlela neutralization, lo mxube ayafukanywa njenge ixesha elicaziweyo liqalise inkqubo ithambe. iziphumo ndlela zovavanyo iyafana.

Ekusebenzeni zonyango lwezilwanyana, isetyenziswa njenge zokuxilonga virus test neutralization reaction oluqhutywa ekuthiwa ubuchule sera ngezibini.

Le ndlela ukuqinisekisa kufunyaniswe isifo wentsholongwane egazini. Ukwenza olu vavanyo isigulana umntu okanye isilwanyana uthathe serum igazi kabini - ekuqaleni kwesifo kunye ngeentsuku 14-21 emva koko.

Ukuba emva kovavanyo lubonise ukwanda isixa antibodies zentsholongwane ngamaxesha-4 okanye ngaphezulu, lo Uxilongo kuqwalaselwa iqinisekisiwe.