ELISA ekuxilongweni

Ifa isifo usulelo - oku ELISA Uhlalutyo, apho liya kusetyenziswa amayeza. Oku kusekelwe ekufumanekeni kwi umguli izilwa-buhlungu okanye nezakhi ezithile ukuchonga arhente causative.

Nezakhi-mzimba zizinto ezo abangena umzimba, ebangela impendulo zikhusela amajoni omzimba ngexesha usulelo iqala ukuvelisa iiproteni ethile - lomzimba.

Wonke umntu iseti eyahlukileyo antigen ka pathogen, yokuyibala, ugqirha unako ukwenza i 'umfanekiso' ukuba Igciwane okukrokrelwayo. Amajoni omzimba kwakhona isebenza emane kwaye ukhupha izilwa ukuba yintsholongwane ethile, yaye aziyi kusebenza kunye nezakhi zezinye kwamagciwane.

Le yindlela apho uxilongo ELISA ekhoyo - esi simo senziwe kwi sakhiwo iantigens omzimba akhiwa lomzimba-iantigens kule simo. Baphi omzimba okanye awakwazi ukwenza ukusabela onjalo? Kakhulu nje, ukuba ziveliswa iindlela zoshishino ngohlobo ngalunye zophando. uhlobo ngalunye antigen enombhalo okanye omzimba athile enzyme izityalo. Ngoko ke, lo ukuyilwa omzimba-iantigens complex, kuya kuba kumafa yayo ilayibhuli, yaye kongezwe yiarhente Umbala, ngenxa yokuba ubukho ileyibhile, zonke izakhiwo ngenxa etshintshe umbala wawo.

Xa isampuli ebanjwe uxilongo ELISA, liphandisisiwe bala izilwa ngamandla kunye awakwazi, ezisetyenziswa ngayo izinto kwesifo kunye nezixhobo ezizodwa liqhuba ukubala inani labo.

ELISA imiphumela

Ukuba awakwazi ziyazibhaqekela usuleleko okanye izilwa kuwo, oko kuthetha ntoni? Oku kuthetha ukuba isigulana iepigenetics usuleleko. Kwaye ELISA Uxilongo ukunika igama ukuya silwanyana ethile linoxanduva imbonakalo le iantigens ngobuciko eliphezulu. Okubangel 'umdla kukuba, xa kuthelekiswa neendlela virological kunye bacteriological loxilongo, ukusebenza kakuhle zentsholongwane akuyiphazamisi kunye nokuzimisela kwakhe.

Kodwa kukho elinye icala le ngqekembe, ukuba uxilongo Ifa afumaneka izoba isampulu, ebonisa ngokucacileyo qhagamshelana yomguli kunye pathogen, oku isenokungabi isiqinisekiso sokuba micro-ephilayo isekhona emzimbeni womntu. Ngapha koko, ii-antibodies inokuba kakhulu ixesha elide emva kokuba ukuchacha emzimbeni wakhe. Isiphumo Ifa engafanele ngetsheki olunye ubukho omzimba, kakhulu, akusoloko oluthembekileyo. Ukwenzela iziganeko omzimba kuthatha ixesha, nto leyo esisizathu sokuba ukuxilongwa zokuphinda-ucalulo lwemizekelo oluqhutywa iiveki ezimbini okanye ezintathu emva yokuqala. Kwaye yiloo nto abaninzi ELISA Diagnostics ze zizifo ezaziwayo iyavakala, hayi kwiiveki ngaphambi kwe ezintathu emva kokosuleleka.

Izinto eziluncedo diagnosis immunoenzymatic kwezifo ezosulelayo

iindlela Uhlalutyo immunofermentatsii lineqela iingenelo, sibonga kuso uye waba ithandwa kangaka kwiminyaka yakutshanje.

Nayo iye yaba lula ukufumanisa uluhlu olubanzi zizifo. Umzekelo, usulelo wentsholongwane egazini (cytomegalovirus,-HIV, i-herpes, hepatitis), uninzi losulelo, ezosulela ngokwabelana ngesondo (mycoplasmosis, negcushuwa, chlamydia, ureaplasmosis), kwakunye nesifo Lyme, kobuchopho, phawula-sithwele kunye nezinye.

Ithuba elifutshane apho kunokwenzeka ukuqhuba isifundo, ingakumbi xa ufuna ukuchonga pathogen okanye iantigens kanye ukuqinisekisa inqanaba omzimba. Njengomgaqo, olu hlobo lokuvandlakanya kufuna umntu iintsuku ezintathu.

ELISA uxilongo ingasetyenziswa kwiimeko apho microscopy okanye ukulinywa kuqulatha ubunzima obukhulu, ukuba ngeke kwenzeke.

Yena uyakwazi ukuvavanya ukusebenza impendulo omzimba isigulane ukuqaliswa gciwane.

Ekubeni kuchongwe umlinganiselo kwiiklasi ezahlukeneyo immunoglobulins, ugqirha uza kukwazi ukubona oko isigaba kungoku nje esi sifo, ukubaluleka kwale nkcazelo ayikwazi nemikhuba.

Zonke ezi eziluncedo baye ELISA Diagnostics abancedisayo ebalulekileyo yesilungu.