Electrophoresis of serum kunye nomchamo. Electrophoresis kweziyi serum: okotshiweyo umthetho

Xa plasma igazi labantu kukho iseti inamacandelo protein. Zahluke kumafa zabo, isakhiwo kunye nokwehla kwindawo ethile, eqhuba yangoku yombane. Kule kokuyilwayo nokwahlukana iiprotini iyonke, leyo localizes ukuya plasma for iinxalenye ezahlukeneyo protein. Ekuqhubeni ii-serum electrophoresis ukufumana ulwalamano lwamanani amacandelo protein ngamnye kunye nezakhiwo. Kuyimfuneko ukuqinisekisa ukuba nesenzeko zabantu ezahlukeneyo lwezidumbu, ezifana usuleleko okanye umhlaza. Kuyinto protein electrophoresis ye serum kubalulekile ekuqhubeni lokufunyaniswa zizifo ezahlukahlukeneyo.

Kakuhle eli indlela

Kuba amaqhezu protein cleavage kusetyenziswa Electrophoresis of serum, umgaqo yayo isekelwe ukuhamba ezohlukeneyo macandelo protein i kwintsimi yombane esabangela. Le ndlela phando ichanekile kakhulu kwaye ulwazi, ngokungafaniyo uhlalutyo igazi standard iyonke. Kodwa lo electrophoresis ibonisa kuphela inani elithile amaqhezu protein, uhlobo kunye nobungakanani inkqubo zophendlo lwezifo ngendlela jikelele. Uhlalutyo uphando ivumela iingcali zonyango ukufumanisa ukuba yintoni na umlinganiselo amaqhekeza protein waphawula ebantwini, yaye zijongisise ezichaphazela ngayo leengxaki ezendeleyo isifo esithile.

Types of amaqhezu protein

Uninzi kulwelo yebhayoloji abantu ephambili okanye igazi, zenza iiprotheni. Imali iyonke eqhelekileyo phakathi 60-80 g / l. Kuba uhlalutyo oluchanekileyo le electrophoresis of serum ephepheni. Olu phando indlela oqhelekileyo uhlalutyo. Eyona medium liphepha filter okhethekileyo. main osemqoka yawo - absorbability eliphezulu. paper ukubamba amanzi ngaphezu ubunzima bayo ngamaxesha 130-200. Kuxhomekeka izixhobo electrophoresis ezisetyenziswa ephepheni ihlala iiyure 4-16. Oku unit kwezakhiwo protein khona. Emva koko, zephetshana iphepha kunyangwa inks ezizodwa ukwenzela uhlalutyo. Le ndlela yeyona ixhaphake kakhulu kwi iilebhu zonyango. Ngenxa kweempembelelo electric current iqhekeza protein, kakubi ityala, lisondela electrode kakuhle ityala. Ngenxa amacandelo protein ke igazi zahlulwe amaqhezu 5 eyaziwa:

• albumin;
• α ₁ globulin;
• α ₂ globulin;
• β - globulin;
• γ-globulin.

Albumins ebizwayo kakubi, ngaba zincinane xa zithelekiswa nezinye ngamaqhezu, ubunzima eziphilayo. Ngenxa kule isantya intshukumo iphezulu kakhulu ngaphezu kwabanye alwayo, kwaye afumaneka Kukude kwindawo yokuqalisa. Owokuqala amaqhezu ezintathu globulin intshukumo ngesantya esisezantsi ngenxa yobunzima balo. Kodwa isantya lincinane ibhalwe γ-globulin. Ezi iiproteni zinzima kwaye becala, xa kuthelekiswa neminye imilinganiselo. isigxina lwabo phantse cala, ngoko le ncindi iproteni akayi itshintshelwe kumgca yokuqala.

nemfuneko yokusebenzisa

Okwangoku, electrophoresis wohlalutyo serum basoloko kuqhutywa moshilongo oluchanileyo sifo. Olu hlahlelo ukuze anikwe njenge therapist, ngoko oogqirha ukuba unciphise iprofayili. Iimpawu ziya kuba phezu Research:

• nezilonda ezahlukeneyo;
• uhlobo ezingapheliyo esi sifo;
• iinkqubo yezifo emzimbeni nokuqina;
• yophela zangaphakathi;
• neoplasms amabi.

Ukulungiselela ukwenziwa uhlalutyo

Ukuze ukufunda ukuziphatha ezi iziphumo ezichanekileyo, iiyure eziyi-8 phambi ukunikela ngegazi kuyimfuneko ukuba bayeke ukutya ulwamkelo. Ukongeza, kuyimfuneko ukuba bavumelane ngokuthatha amayeza, ukuba ikho, kunye ugqirha khona.

Zesimo igazi

Kuba ziphumo zabaxwa ngempazamo, kuyimfuneko ukunciphisa amathuba igazi ekunqandeni ukuba isigqibo amaqhezu protein ziiprotein iyonke. Electrophoresis of serum igazi wenziwe ngocoselelo, njengoko kukho amathuba okuba uhlaba iziphumo ngenxa lwefibrinogen. Angakwazi ukufihla iiproteni ezingaqhelekanga okanye kubhidaniswe nabo.

izinto eziqhelekileyo

Kwisithuba seentsuku emva komhla isampuli sebelungele uvavanyo ukuze electrophoresis kweziyi serum. UNorma wafumana iindidi yokusebenza abadala:

1. Iyonke protein - 63-82 g / l.
2. Albumin - 40-60% yenani lilonke zamaqhezu.
3. α-globulin ₁ - 2-5%.
4. _{α-globulin-2} - 7-13%.
5. β-globulin - 8-15%
6. γ-globulin - 12-22%.

Kufuneka bahlalutye

Ukutshintsha Isixa sayo nayiphi na iqhezu protein kwi phezulu okanye ezantsi asenokubonisa ukuba uphuhliso ngezifo ethile. Ukuze ufumane inkcazelo enokuthenjwa oku kufuneka electrophoresis kweziyi serum. iziphumo kokuhlaziya lula iingcali zonyango Uxilongo kunye nokukhetha konyango.

Ukunyuka albumin

Ekuqaleni, ukuqinisekisa ubungakanani albumin kwi ucazululo lweziphumo. Ukwanda kule iqhezu ukuthetha ngayo emzimbeni. Oku kunokwenzeka ukuba isigulane sifumana ukugabha elide okanye ukuphazamiseka kwinkqubo yokwetyisa. Albumin kwakhona kwandisa yenzeka ukutsha kwindawo enkulu ulusu.

albumin Ukuncipha

Eziyingozi kakhulu, ukuba umzimba unciphisa isixa albumin, kusenokuba Bothetha ezimbi zilandelayo:

1. Ukuphazamiseka ze izintso wesibindi.
2. Pathology ye phecana zesisu.
3. iinkqubo Infectious.
4. Yokuphazamiseka nemithambo yegazi.
5. Ukopha.
6. Umhlaza.
7. Nokudumba.
8. Rheumatism.

A ukuncipha umlinganiselo albumin nako:

1. Xa oomama abakhulelweyo.
2. Ngeedosi ezingaphezu iziyobisi.
3. Ngexesha fever elide.
4. Xa iintshayi enzima.

Ukutshintsha inani α1-globulin

Ukunciphisa ubungakanani A1-globulin ebhalwe kunye ukuswela α ₁ -antitrypsin. Ukwandisa ingongoma enye exacerbation of ukudumba emzimbeni, ukuphazamiseka kwesibindi kwindawo ukubola kwithishyu.

Ukunciphisa α2-globulin

Bhalisa kwi seswekile, nangokurhala zepancreas, xa kuzalwa kunye senyongo kwi hepatitis imvelaphi ityhefu. Oku kubonisa ukuba ayilunganga, nezingafezekanga.

Ukunyuka α2-globulin

Oku kwenzeka phantsi kwale miqathango ilandelayo:

1. Inflammations, ngokukodwa phambi exudates ubovu (ukukrala kwemiphunga kunye nezinye iinkqubo kunye ubukho ubovu).
2. ukuphazamiseka kwizicwili (umz, isifo samathambo).
3. Umhlaza.
4. Amaxesha alulame emva nokutsha.
5. Kidney ukubandakanyeka.

Ukongeza, loo simanga uphawu igazi hemolysis in vitro ngexesha sifundo.

Ukwanda β-globulin

Hyperlipidemia kuboniswe (ukwandisa isixa lipids egazini), wesibindi nezintso ngezifo. Kungabonwa ne esuswini evulekileyo, kunye hypothyroidism (thyroid umsebenzi disorder). amaqhezu okwehlileyo iliso kwi gipobetalipoproteinemii (ukunyuka egazini icandelo betalipoprotein).

Utshintsho kwi iqhezu γ-globulin

Oku kubambene iquka e immunoglobulins kwamalungu ayo. Ngoko ke ukwanda γ-globulin ebhalwe ukwehluleka nezifo. Oku kwenzeka xa iintlobo zosulelo, uphuhliso inkqubo kwiintetho ezikralayo, itshintsha izicwili niyitshise ngomlilo iingozi. Ukukhula γ-globulin babonakala kwizigulane kunye kohlobo. Phantse imeko efanayo uphawu ngokoma. Kwiimeko kakhulu kwesi sifo isixa kwiproteni γ-globulin iqhekeza ngaphezulu kakhulu kunokuba albumin. Phantsi izigulo ezithile kuwa ukuyilwa γ-globulin, kwaye uphuhliso iiprothini kwalungiswa egazini - paraprotein. immunoelectrophoresis - izifundo kwenziwa ukuba ukujonga uhlobo a phuhliso. Le ndlela kuvamile ukuba sisifo ezigula Waldenstrom kunye ngezifo.

Ukwandisa inani γ-globulin nazo ezendeleyo ezimbi zilandelayo:

• lupus zomzimba;
• endotelioma;
• Ifomu isifo samathambo;
• osteosarcoma;
• leukemia lymphocytic ezingapheliyo;
• candidiasis.

Ukunciphisa isixa γ-globulin

Ukuhla kwi γ-globulin ziye zohlulwa ngokwendidi 3:

1. Saline (okufana abantwana ukusuka kwiinyanga ezintathu ukuya kwezintlanu).
2. Naso (asakhasayo emva kokubeleka).
3. Idiopathic (nini azinako ukusekwa unobangela kuphuhliso).

ukuncipha Secondary ebhaliswe kuphuhliso izifo ezibangela zokuncipha yamajoni omzimba. Kutsha yezonyango kusiba nohlahlelo ukufumanisa ubungakanani prealbumin. Ngokuqhelekileyo, isifundo esinjalo olwenziwe izigulane aba labagula kakhulu.

Ukunciphisa inani prealbumin kubaluleke kakhulu yaye ichanile uvavanyo ukujonga yimibutho protein ukuhluleka umzimba umguli. Ngexesha uhlalutyo lwenziwa ulungiso prealbumin protein yokucolwa kwezi izigulane.

umchamo electrophoresis

Umgaqo azihlole iyafana esenza electrophoresis kobuchwepheshe serum. Ithwalwa phandle ukuxilongwa okanye ubhaqo ezinye ukungaqheleki. Ukongeza, azihlole kuya kunceda ukuchonga isigulane ubukho proteinuria.

isiphelo

Electrophoresis of serum kunye nomchamo - iindlela ezibalulekileyo lokufunyaniswa zizifo ezahlukahlukeneyo ezosulelayo. Ngenxa indlela zophando kunye nokuchaneka, baya kukunceda ukuba ukujonga uhlobo ngezifo. ukuxilongwa - indlela Qinisekisa ukuba balungise unyango kunye bachache ngokupheleleyo.