Microscopy ezibengezelayo: features uphando

Igama elithi "microscope" nesinempandla zamaGrike. Xa translation kuthetha isifundo bezinto kunye nezixhobo eliphezulu-ngqo. . Kwiminyaka yakutshanje baba kuthandwa ezibengezelayo kunye electron microscopy.

isisombululo

Phantsi kwalo, njengokuba umthetho, baqonde kumgama omncinane apho inokuba izinto distinguishable ngokucacileyo. Kwi isisombululo amandla weqondo izixhobo sokungena iya kuxhomekeka kwihlabathi kakhulu, uyakwazi ukubona ubungakanani i element. Ekubeni kukho ukwanda kakhulu kwizibonelelo umda luyakwazi ukudibanisa. Ngako oko, kukho imida ethile, ngaphezu apho indlela eziqalelo ingamampunge.

Indlela fluorescence microscopy: indlela

Xa conversion energy i ethintela into ibe radiation ebonakalayo kwenzeka kukhanya. Ibizwa ngokuba chweba. Le meko kungenxa yokuba kwezinye izinto phantsi kwempembelelo ukukhanya aqale bukhazimle kwezinye ngamaza (idla enkulu). Kwakhona, ezinye izinto ukuba kwiimeko zokukhanyisa eziqhelekileyo elikhankanyiweyo umbala phantsi kwempembelelo ukukhanya yelanga itshintsha umbala wayo.

Okukodwa

An into ezingenako endibubonileyo phantsi ukukhanya UV, uyakwazi kukhutshwe ayenechaphothi eqaqambileyo, ukuba iphathwa na into ekhethekileyo. Xa izinto zonke imibala ezahlukeneyo ukukhanya ebumnyameni. ngamandla linobuqaqawuli yahlukile, kodwa umthetho, mncane. Kulo mba, fluorescence microscopy iyasebenza egumbini mnyama. Xa usebenzisa olu hlobo uphando kungabonwa ekukhanyeni into, ngawo imitha. Le khemikhali Ukwakheka izicwili, iiseli kuya kuchaphazela umgangatho sifundo. Fluorescence microscopy kuthathwa ukuya kumlinganiselo othile isifundo histochemical.

ngezigaba

может быть первичной или вторичной. Fluorescence microscopy ingaba lokuqala okanye ezenqanaba lesibini. Kwimeko yokugqibela, injongosenzi kusetyenzwa yi iikhompawundi ezikhethekileyo ngokunika chweba. основывается на собственной способности исследуемого элемента излучать свет. microscopy ezibengezelayo Primary zisekelwe ekukwazini yabo an element ukuze kukhutshwe ukukhanya.

izixhobo

Fluorescence microscopy lwenziwa kusetyenziswa iintlobo kwezixhobo. Le illuminator lisebenza ingundoqo element. Lixhotyiswe isibane UV. Ukongeza, ifowuni isebenzisa uluhlu lwamacebo okucoca ulwelo. Kwezinye izixhobo kakhulu izinto ezimbalwa sisimo ezahlukeneyo. Kuxhomekeke apho mbala usetyenziswa chweba excitation - ultraviolet okanye aluhlaza, phakathi umthombo wokukhanya kunye into phantsi kophando obekwe i sihluzi esisiso esifumanekileyo. Ekubeni chweba element ngemikroskopu ngenkuthalo buthathaka ukukhanya excitation, iya kubanjwa kuphela kwi imeko enye. Intsalela semitha uqhebeke ukusuka kumthombo ukuya kunqunyulwa lokucoca omthubi noluhlaza. Ibekwe phezu eyepiece isixhobo. Isiphumo chweba kakhulu iqapheleka kuya kwenzeka xa lokucoca ziguyiwe ngokupheleleyo kude imisebe ephumayo ukukhanya kumthombo.

Yintoni ufakelo ekukhanyeni ebonakalayo? Kuyo kukho umthombo ukukhanya okuqaqambileyo kunye ngemicroscope eziphilayo. Phakathi iyunithi esipilini isibane enyusiweyo eliluhlaza-Violet lokucoca ulwelo. Oku ingaba FS-1, eUFS-3 njalo njalo. isihluzi Yellow esingena phezu eyepiece le ngemicroscope. Ngoncedo lwabo, into owa ukukhanya blue-Violet. Yena chweba yimincili. Kodwa oku kukhanya kuphazamise ukubonisa ukukhanya. Ngoko ke, endleleni eya iliso yakhe kuba wayenze isifungo lokucoca etyheli. Ukufakelwa kwezibane eyenziwe indlela Koehler, ngaphandle mnye. Uvalo ekondensa kufuneka ibe ivuliwe ngokupheleleyo. Xa isifundo kubalulekile ukuba usebenzise non-flourescent oyile emanzini. Ukuze kucutheke ukukhutshwa elikhethekileyo idityaniswa noko nitrobenzene (2-10 kusithwa / 1 g).

Ezibengezelayo microscopy: zokusebenza eziphilayo

Izinto eziluncedo ze olu hlobo phando zezi:

1. Ukuphandwa ngokufumaneka imifanekiso umbala.
2. high umehluko self-kubacacisela izinto kwisiqalo emnyama.
3. Bangabonwa zalapha kweentlobo ezithile iintsholongwane kunye iintsholongwane.
4. Ithuba lokufunda izinto eziphilayo selubala kunye zimnyama.
5. Uphononongo wobomi iinkqubo Dynamics zabo.

Kuyimfuneko ukuba bathi microscopy ezibengezelayo ikhuthaza iindlela ubalaseleyo histology kunye cytochemistry, ngokukhawuleza.